The 6 X histidine motif is used as a tag on many recombinant proteins to facilitate purification.It can then be purified using immobilized metal ions,traditionally nickel,in a procedure termed immobilized-metal affinity chromatography.Further analysis of the protein can be achieved using an antibody specific to the 6 X histidine in techniques such as western blotting.The antibody recognizes the His-tag fused to targeted proteins in transfected or transformed cells. |
Specification | |
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Aliases | 6 His tag; HHHHHH tag |
clone | 6A8H8 |
Host/Isotype | Mouse IgG1 |
Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
Immunogen | Synthesized peptide of His Tag (AA: HHHHHHHH-KLH). |
Formulation | Purified antibody in PBS with 0.05% sodium azide |